Journal: Diversity

Article Title: Halomonas kashgarensis sp. nov., a Novel Species Isolated from the Rhizosphere Soil of Phragmites australis (Cav.) Trin. ex Steud in Kashgar County, Xinjiang, China

doi: 10.3390/d17020098

Figure Lengend Snippet: Figure 1. The ML phylogenetic tree based on the 16S rRNA gene sequences showing the phylogenetic relationship of strain zp-37T and other closely related members of the genus Halomonas. Bootstrap support values were calculated from 1000 replicates (only values ≥70% were shown). The bold font represents the novel species identified in this study. GenBank accession numbers were provided in parentheses for reference. Carnimonas nigrificans CTCBS1T was used as the outgroup.

Article Snippet: Up to now, December 2024, the genus Halomonas includes 144 species with valid publications (https://lpsn.dsmz.de/search?word=Halomonas, accessed on 27 December 2024).

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Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Isolation and initial characterization of Halomonas species. (a) Point of sample collection, marked on a map of the Great Salt Lake, UT, USA. Specifically, samples were taken off the Spiral Jetty near Rozel Point at coordinates 41°26′15.5″N 112°40′09.7″W. Imagery ©2024 Terra Metrics, Map data ©2024 Google. (b) Microscopy of Halomonas species with fluorescence staining of intracellular polyester granules. (c) Growth curve of isolated Halomonas species on rich complex medium (aerobic, 30°C, 1 M NaCl).

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: Isolation, Microscopy, Fluorescence, Staining

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Phylogenetic affiliation and neighborhood of Halomonas sp. CUBES01 based on 16S rRNA sequence comparison in the form of a phylogenetic tree based on the minimum evolution method.

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: Sequencing, Comparison

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Phylogenetic affiliation and neighborhood of Halomonas sp. CUBES01 based on 16S rRNA sequence comparison in the form of a heatmap of the pairwise 16S rRNA gene sequence distance of CUBES01 to other strains. The scale indicates the fraction of non-identical base pairs between the compared sequences. GenBank IDs for each Halomonas species can be found in Table S7.

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: Sequencing, Comparison

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Circular representations of the Halomonas sp. CUBES01 chromosome displaying relevant genome features; yellow/orange: A-T skew; blue/red: C-G skew; green: +CDS regions; pink: −CDS regions.

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Features of the Halomonas sp. CUBES01 genome and coding sequences

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Growth of Halomonas sp. CUBES01 on different substrates (equivalent C-mol quantities of all substrates except for NB). The blue graphs represent the growth curves while the green graphs represent the pH throughout the cultivation. The exponential phases used to calculate growth rates are indicated by red dashed lines. Sampling points for quantification of PHB content are represented by square (early exponential), cross (late exponential), and triangle (stationary) symbols and directly correspond to the data shown in . The same sampling points were also used for end-product analysis.

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: Sampling

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Accumulation of PHB by Halomonas sp. CUBES01 in early exponential (blue), late exponential (ochre), and stationary (turquoise) growth phase on different substrates, directly corresponding to the sampling points indicated in . While the main chart reflects the qualitative PHB content dependent on the growth stage [inferred by fluorescence intensity of whole cells stained with Nile red and normalized to optical density (OD)], the inset indicates the quantitative per-biomass PHB yield (obtained gravimetrically, late exponential and stationary phase combined). SUC, sucrose; GLC, glucose; FRC, fructose; GLY, glycerol; PRP, propionate; ACT, acetate; GlcN, glucosamine; AcGlcN, acetyl-glucosamine.

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: Sampling, Fluorescence, Staining

Journal: Applied and Environmental Microbiology

Article Title: Isolation and characterization of a Halomonas species for non-axenic growth-associated production of bio-polyesters from sustainable feedstocks

doi: 10.1128/aem.00603-24

Figure Lengend Snippet: Estimated biomass-specific maximum PHB production rates of Halomonas sp. CUBES01. Data based on rates of biomass formation during exponential growth and PHB content during late exponential phase. Data correspond to values shown in .

Article Snippet: The Halomonas strain was deposited to the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) GmbH at the Leibnitz Institute (Braunschweig, Germany) under the designation CUBES01 and is available under accession number DSM 115203.

Techniques: